Bacteriological and Physicochemical Assessment of Akwaka River in Port Harcourt Nigeria Receiving Abattoir Effluent During Dry Season
Keywords:
Bacteriological, Physicochemical, Lipolytic, Cellulolytic, Amylolytic, ProteolyticAbstract
A total of 50 samples were collected from five different locations (stations) along the River. The media used were nutrient agar, MacConkey agar, Eosine methylene blue agar, Salmonella-Shigella agar, proteolytic medium, Lipolytic medium, Amylolytic medium and cellulolytic medium. The method of isolation was pour plating technique. Colonial morphology, gram staining and biochemical tests were carried out using the standard microbiological methods. The results were subjected to statistical analysis- ANOVA. The
mean count in log10cfu/ml of TAPC ranged from 4.04±1.0 – 6.25 ± 5.2; Escherichia coli count, 1.84 ± 1.0 – 4.15 ± 1.62; coliform count, 2.0 ± 1.8 – 4.06 ± 1.2; Salmonella- Shigella count, 1.01 ± 0.2 – 1.35 ± 1.0; proteolytic count, 0.52 ± 0.3 – 1.98 ± 1.0; amylolytic count, 0.76 ± 0.1 – 1.15 ± 1.03; cellulolytic count, 0.44 ± 0.2 – 1.01 ± 0.4; lipolytic count, 0.43 ± 0.3 – 1.05 ± 0.23.The bacteria isolated and the percentage occurrence were Bacillus species, 13%, Salmonella species, 13%,shigella species 8.5%, Pseudomonas specie 8.5%, Micrococcus species 12.3%, Staphlococcus auerus 16.2%, Klebsiella species 13.9%, and E.coli14.6%. Temperature, PH, conductivity, total dissolved solid. Biochemical oxygen demand, Chemical Oxygen demand and Sulphate were not significantly higher. Phosphates were significantly higher. This study revealed that the river is heavily contaminated and toxic.
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References
Ademoye, A. O, Opere,B.OO and Makinde,S.C.O (2006). Microbial Content of Abattoir and it’s Contaminated soil in Lagos, Nigeria. African Journal of Biotechnology vol. 5(20), pp1963-1968.
Adeoye, A. (2007). Medical Laboratory Practice 1sted. Femco Publishers Limited, Lagos; 153.
Adeyemi, I. G. and Adeyemo (2007). Waste Management Practices at the Bodija Abattoir, Nigeria, International Journal Environmental studies 64(1): 71-82.
Adeyemi- Ale, O.A(2004). Impact of abattoir effluent on the physicochemical parameter of Gbagi stream (Ode-eran) Ibadan, Nigeria. Ilor Jour Sci 1(1):100-109.
Agwung, D. (2007). Research Orientation and Thesis Compilation; a guide for Biological and Health Sciences. Ark of Wisdom Publishers, Aba, Nigeria, 172-183.
Akpor, O.B and Muchie, M (2011). Environmental and public health implication of waste water quality. African Journal of Biotechnology; 10(13); 2379-2387.
Alonge, D. O. (1991). Textbook of Meat Hygiene in the Tropics. Farmcoe Press, Ibadan; 58.
Cheesbrough, M. (2005). District Laboratory Practice in Tropical Countries. Cambridge University Press, United Kingdom, 157-187.
Chukwu, O.E. S. Ajisegiri, K. R. Onifade and O.D. Jimoh (2007). Environmental Impact on Auditing of food Processing Industry in Nigeria.The case study of climate and air Quality; 11(2) 77-85.
Eze V. C. and G. C. Okpokwasili (2010). Microbial and other related changes in a Niger Delta River Sediment receiving in industrial effluents. Continental Journal of Microbiology; 4:15-24.
Eze, V. C. and E. P. Ikeri (2010). Enumeration and Characterization of Microorganisms involved in the degradation of abattoir waste in Port Harcourt. International Journal of Current Research; 6:53-57.
Joanne, M.W, Linda, M.S, and Christopher, J.W (2011). Influence of Environmental factors on growth. Prescott ‘s Microbiology,8th ed.
Mcgraw Hill New York, 177-178.
Meadows, R. (1995). Livestock Legacy; Environmental Health Perspectives: 103;(2): 1096-1100.
Moe, C. L. (1997). Waterborne Transmission of Infection Agents in Manual of Environmental Microbiology. C. J. Hurst, G. R. Knudsen, M. J. Mohorney, L. D. Stetzenbach and M.V. Water (Eds). A. S. M. Press Washington D.C.Nester, E. W., Anderson, D. G., Evans, C. R., Pearsall, N. N. and Nester; M. J. (2004). Microbiology: A human perspective, 4th edition, McGram-Hill; New York: 11-20
Ochei, J. O. and A. A. Kolhatkar (2007). Medical Laboratory Science: Theory and Practice, Tata McGraw-Hill Publishing Company Limited, New York: 637-655, 700-745.
Ogbonnaya, C. (2008). Analysis of ground water pollution from Abattoir Waste in Minna. Nigeria Research journal of Diary Science. 2(4):74-77.
Okoli, C. (1991). Encyclopedia of Microbiology. ABIC publishers Enugu, Nigeria; 155.
Okonko,I.O., Adejoye, O.D., Ogunnusi,T.A, Fajobi ,E.A and, O.B. Shittu (2008). Microbiolgical and Physicochemical analysis of different water samples used for domestic purposes in Abeokuta and Ojota, Nigeria. African Journal of Biotechnology, 7(3):617-621.
Olaiya, S, Mahmud,H., Eboreime, L and O.C. Afolabi (2016). Physicochemical and Microbial analysis of the effects of Abattoirs operation in Estako West and Central, Edo State, Nigeria. Global Journal of Science Frontier Research Environmental and Earth Science vol. 6(3).
Pelzer, M. J., E.C.S. Char, and N. R. Krieg, (1993). Microbiology Concept and Application revised edition, McGraw-Hill, New York; 821-825.
Piet, K. (2009). Waste Disposal Technology. Mpumalanga. South Africa; 24-27.
Pink, D. H. (2006). Investing in Tomorrow’s Liquid Gold. Retrieved on 7th March, 2009, from www.soilassociation.org
Prescott, N. A. and J. P. Harley (2008). Microbiology 7th edition McGrawHill, New York; 1054-1060.
Ram, S. L, Pravin, U.S and S. P. Deepali (2011). Pollution in water of Kasardi River flowing along Taloja Industrial Area of Mumbai, India, World Environment, 1(1),6-13.
Reston, V. A. (2001). A Primer on water Quality. Retrieved on 11th March, 2009, from www.unichedu/ggs 265/society water pollution wtm.
Schueler, R. (2000).Microbes and urban watersheds concentration, sources and pathway. Reprinted in the Practice of Watershed Protection, Center for watershed protection. Retrieved on 11th march, 2009, from www.nrdc.org/water/pollution/storm/chapz.asp.
Shaheen, E. I. (1992). Technology of Environmental Pollution and Control (2nded.). Pennwell Publishing company, Spain. 360-373.
Sneath P. H., Mair, N. S., Sharp, M. E. and J. G. Holt (1994). Berge’s manual of determinative Bacteriology 2 Williams and Wilkins, Baltimore.
Sobukola, O.P, Awonorin, O.S, Idowu, A.M and O.F. Bamiro (2009). Microbial profile and critical control points during processing of “robo” snack from melon seed (Citrullus lunatus thumb) in Abeokuta, Nigeria. African Journal of Biotechnology8(10); 2385-2388.
Tennessce Department of Environment and Conservation, (2002). Tennessee Erosion and sediment control. Retrieved on 7th march, 2009, from www.youtube.com.
United State Environmental Protection Association (1987). Protecting water quality for Agriculture Runoff. Retrieved on 7th march, 2009 from www.nrdc.org/water/pollution/storm/chapz.asp
West, L. (2006). World Water Day; A Billion People Worldwide Lack Safe Drinking water retrieved on 7th march 2009, from www.youtube.com.
Williams, E. E. and M Demello (2007). Why Animals Matter. AK Press, New York; 73.
World Health Organization (WHO), (2004). Guidelines for Drinking water quality, WHO, Geneva
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