Bacteriological and Physicochemical Assessment of Akwaka River in Port Harcourt Nigeria Receiving Abattoir Effluent During Dry Season


  • O.U. Manu Department of Microbiology, Madonna University Elele Author
  • V.C. Eze Department of Microbiology, Michael Okpara University of Agriculture, Umudike. Author
  • B.C. Uzor Department of Microbiology, Madonna University Elele Author


Bacteriological, Physicochemical, Lipolytic, Cellulolytic, Amylolytic, Proteolytic


A total of 50 samples were collected from five different locations (stations) along the River. The media used were nutrient agar, MacConkey agar, Eosine methylene blue agar, Salmonella-Shigella agar, proteolytic medium, Lipolytic medium, Amylolytic medium and cellulolytic medium. The method of isolation was pour plating technique. Colonial morphology, gram staining and biochemical tests were carried out using the standard microbiological methods. The results were subjected to statistical analysis- ANOVA. The 
mean count in log10cfu/ml of TAPC ranged from 4.04±1.0 – 6.25 ± 5.2; Escherichia coli count, 1.84 ± 1.0 – 4.15 ± 1.62; coliform count, 2.0 ± 1.8 – 4.06 ± 1.2; Salmonella- Shigella count, 1.01 ± 0.2 – 1.35 ± 1.0; proteolytic count, 0.52 ± 0.3 – 1.98 ± 1.0; amylolytic count, 0.76 ± 0.1 – 1.15 ± 1.03; cellulolytic count, 0.44 ± 0.2 – 1.01 ± 0.4; lipolytic count, 0.43 ± 0.3 – 1.05 ± 0.23.The bacteria isolated and the percentage occurrence were Bacillus species, 13%, Salmonella species, 13%,shigella species 8.5%, Pseudomonas specie 8.5%, Micrococcus species 12.3%, Staphlococcus auerus 16.2%, Klebsiella species 13.9%, and E.coli14.6%. Temperature, PH, conductivity, total dissolved solid. Biochemical oxygen demand, Chemical Oxygen demand and Sulphate were not significantly higher. Phosphates were significantly higher. This study revealed that the river is heavily contaminated and toxic.


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How to Cite

Manu, O., Eze, V., & Uzor, B. (2024). Bacteriological and Physicochemical Assessment of Akwaka River in Port Harcourt Nigeria Receiving Abattoir Effluent During Dry Season. Toxicology Digest, 4(1), 33-44.