Toxicological Assessment of Zingiber officinale Roscoe (Ginger) Root Oil Extracts in Albino rats


  • Esther O. Idang Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, PMB 1017 Uyo, Nigeria Author
  • Omoniyi K. Yemitan Department of Pharmacology, Therapeutics and Toxicology, Lagos State University College of Medicine, Lagos, Nigeria Author
  • Herbert O. C. Mbagwu Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, PMB 1017 Uyo, Nigeria Author
  • Godswill J. Udom Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, PMB 1017 Uyo, Nigeria Author
  • Emmanuel O. Ogbuagu Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Medicine, Abia State University, Uturu, Nigeria Author
  • John A. Udobang Department of Clinical Pharmacology and Therapeutics, Faculty of Clinical Sciences, University of Uyo, PMB 1017 Uyo, Nigeria Author


Ginger oils, histopathology, essential oils, oxidative stress, reversibility studies


Zingiber officinale Roscoe root is a medicinal plant popularly used to relieve symptoms of nausea/vomiting associated with motion sickness, surgery and pregnancy. Study was to screen the continuous use of the root oil extracts of ginger for any undesirable outcome in rodents. Acute toxicity was conducted in mice using OECD425 procedure. 50 male Wistar rats were randomly divided into five groups (10/group) and orally-treated daily, thus: Group I–Control (corn oil-0.5 mL/kg), Groups II-V received ZOF and ZOE (0.02, 0.002, 0.4 and 0.04) mL/kg body weight, respectively. Animals (6/group) were euthanized under diethyl ester and sacrificed. Blood samples were collected by cardiac-puncture for biochemical analysis. Vital organs were eviscerated, blotted/weighed and fixed in 10% formalin for histopathological assessment. Other animals (4/group) were retained for reversibility studies. Results presented significant(P<0.05) increased kidneys, lungs, liver and spleen weights at low doses of ZOF respectively. Increased ALT, decreased ALP and AST at low doses of ZOF and ZOE were respectively recorded. Malondialdehyde increased at low dose of ZOF. 
Histopathology of liver and spleen showed forms of pathology. Besides MDA, test effects were reversed on extract discontinuation. Results indicate that long-term use of ZOF may induce some undesirable effects like oxidative stress amongst others. Findings suggest caution on chronic use of ginger oils


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Author Biography

  • Godswill J. Udom, Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, PMB 1017 Uyo, Nigeria

    Gimmex Health Consult, Suites B6 Real Towers Complex 26 Ekukinam Street, Utako District, Abuja, Nigeria


Abdelhalim, M. A. K. and Moussa, S. A. A. (2013). The biochemical changes in rat’s blood serum levels exposed to different gamma radiation doses. African Journal of Pharmacy and Pharmacology, 7(15): 785 – 792.

Abdullah, S., Abidin, S. A. Z., Murad, N. A., Makpol, S., Ngah, W. Z. W. and Yusof, Y. A. M. (2010). Ginger extract (Zingiber officinale) triggers apoptosis and G0/G1 cells arrest in HCT 116 and HT 29 colon cancer cell lines. African Journal of Biochemistry Research, 4(4): 134 –142.

Altman, R. D. and Marcussen, K. C. (2001). Effects of ginger extract on knee pain in patients with osteoarthritis. International Journal of Arthritis and Rheumatism, 44(11): 2461 –2462.

Bessey, O. A., Lowry, O. H. and Brock, M. J. (1946). A method for the rapid determination of alkaline phosphate with fibecubic millimeters of serum.Journal of Biological Chemistry, 164: 321 – 329.

Cowell, D. C., Dowman, A. A., Lewis, R. J., Pirzad, R. and Watkins, S. D. (1994). The rapid potentiometric detection of catalase positive microorganisms. Biosens

Bioelectron, 9: 131 – 138.

Davey, M. W. I., Stals, E., Panis, B., Keulemans, J. and Swennen, R. L. (2005). High-throughput determination of MDA in plant tissues. Analytical Biochemistry,

(2): 201 – 207.

Douredjou, P. and Koner, B. C. (2008). Effect of different cooking vessels on heat – induced lipid peroxidation of different edible oils. Journal of Food Biochemistry, 32: 740 – 751.

Eleazu, C. O., Iroaganachi, M., Okafor, P. N., Ijeh, I. I. and Eleazu, K. C. (2013). ameliorative potentials of ginger (Z officinale Roscoe) relative organs weights in streptozocin induced diabetic rats. International Journal of Biomedical Science, 9(2): 82 – 90.

Eru, M., Eru, K. E., Obeten, K. C., Uruakpa, and Mesembe O. E. (2014).Histological evaluation of the combine extracts of aqueous Zingiber officinale (ginger) root and

honey on the stomach of adult Wistar rats. Global Journal of Biology, Agriculture and Health Sciences, 2(4): 43-45.

Farah, A. O., Nooraain, H., Noriham, A., Azizah, A. H. and Nurul, H. R. (2013). Acute and oral subacute toxicity study of ethanolic extract of Cosmos caudatus leaf in Sprague Dawley rats. International Journal of Biosciences, Biochemistry and Bioinformatics, 3(4): 301 – 305.

Farmer, E. E. and Davoine, C. (2007). Reactive electrophile species. Current Opinions in Plant Biology, 10(4): 380 – 386.

Gilani, A. H. and Rahman, A. U. (2005). Trends in ethnopharmocology. Journal of Ethnopharmacology, 100: 43-49.

Goudah, A., Abo-EL-Sooud, K. and Yousef, M. A. (2015) Acute and subchronic toxicity assessmentmodel of Ferula assa-foetida gum in rodents. Veterinary World, 8(5): 584

- 589.

Gupta, L. M. and Raina, R. (1998). Side effects of some medicinal plants. Current Science, 75(9): 897 – 900.

Newman, D. J. and Price, C. P. (1999). Renal Function and Nitrogen Metabolites. In: Burtis, C. A. and Ashwood, E. R. (eds.), Tietz Textbook of Clinical Chemistry. 3rd

Edition. W. B. Saunders Company, Philadelphia, 1204p.

NIH (1996). Guide for the Care and Use of Laboratory Animals. NIH Publication No. 85 – 23.

OECD (2001). Guidance Document on the Recognition, Assessment and Use of Clinical Signs as Humane Endpoints for Experimental Animals Used in Safety Evaluation. Environmental Health and Safety Monograph Series on Testing and Assessment No 19.

Ohkawa, H., Ohishi, N. and Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry,95: 351 – 358.

Ping, K.Y., Darah, I., Chen, Y., Sreeramanan, S. and Sasidharan, S. (2013). Acute and subchronic toxicity study of Euphorbia hirta L. methanol extract in rats. Biomedical Research International, 2: 1 - 14.

Riede, U. and Werner, M. (2004). Color Atlas of Pathology. Thieme Medical Publishers, New York, 426p.

Reitman, S. and Frankel, S. (1957). Glutamic-pryuvate transaminase assay by colorimetric method. American Journal of Clinical Pathology, 28: 56.

Sainz-Perez, A., Gary-Gouy, H., Portier, A., Davi, F., Merle-Beral, H., Galanaud, P. and Dalloul, A. (2006). High MDA-7 expression promotes

malignant cell survival and p38 map kinase activation in chronic lymphotic leukemia. Leukemia, 20: 498 – 504.

Sakr, S. A., Lamfon, A. H. and Essawy, A. E. (2011). Ginger (Zingiber officinale) Extract ameliorates metalaxyl fungicide induced nephrotoxicity in albino mice. African Journal of Pharmacy and Pharmacology, 5: 104 – 112.

Singh, G., Kapoor, I. P. S., Singh, P., Heluani, G. S. D. and Lampasona, M. P. D. (2008). Chemistry, antioxidant and antimicrobial investigations on essential oil and

oleoresins of Zingiber Officinale. Journal of Food and Chemical Toxicology, 46: 3295 – 3302.

Stoilova, I., Krastanov, A., Stoyanova, A., Denev, P. and Gargova, S. (2007): Antioxidant activity of a ginger extract (Zingiber officinale). Food Chemistry, 102: 764 – 770.

Sun, Y., Oberley, L. W. and Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical Chemistry, 34: 497 – 500.

Tiedge, M., Lortz, S., Drinkgern, J. and Lenzen, S. (1997). Relation between antioxidant enzyme gene expression and antioxidant defence status of insulin-producing cells. Diabetes,46: 1733 – 1742.

WHO (1997). Monographs on Selected Medicinal Plants. Unpublished Document.

Yemitan, O. K., Adeyemi, O. O., and Izegbu, M. C. (2015). Toxicological and reversibility assessment of dalbergia saxatilis root extracts on body and organ weights, hepatic functions and peroxidation in rats. European Journal of Medicinal Plants, 11(4): 1 – 13.

Young, H. Y., Luo, Y. L., Cheng, H. Y., Hsieh, W. C., Liao, J. C. and Peng, W. H. (2005). Analgesic and antiinflammatory activities of [6]-gingerol. Journal of Ethnopharmacology, 96: 207 – 210.







How to Cite

Idang, E. O., Yemitan, O. K., Mbagwu, H. O. C., Udom, G. J., Ogbuagu, E. O., & Udobang, J. A. (2024). Toxicological Assessment of Zingiber officinale Roscoe (Ginger) Root Oil Extracts in Albino rats. Toxicology Digest, 4(1), 108-119.


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