Diabetes-Induced Cortical Neural Damage in Male Wistar Rats: Ameliorative Role of a Flavonoid
Keywords:
cortical neural damage, diabetes mellitus, diabetic neuropathy, polyphenols, antioxidant, oxidative stressAbstract
Diabetes mellitus (DM) is a multifactorial metabolic disease characterized by persistent hyperglycaemia associated with numerous toxic outcomes including neuropathy amongst others. Naringenin (NG) is a flavonoid with diverse pharmacological spectrum. This study evaluated the ameliorative role of naringenin against streptozotocin–induced diabetic cortical neural damage in rats. Post induction of diabetes, 30 Wistar rats were randomly allotted to five groups of six each and orally treated daily thus: Group-1 (vehicle control) received 0.1 mol/L citrate buffer, group 2 (negative control), groups 3-5 received 25, 50 and 100 mg/kg body weight of NG respectively for 35 days during which their fasting blood glucose (FBG) levels were measured. On the 37th day, all animals were sacrificed under diethyl ether anaesthesia, cranial vaults were opened and cerebral cortex eviscerated and suspended in liquid nitrogen for 5 min until all particles were frozen. Samples were stored in freezer at -80 0C for tissue enzymatic studies. At 50 and 100 mg/kg NG, significant (P<0.05) dose-dependent reduction in FBG as well as elevated serum insulin levels at 100 mg/kg NG only were recorded. Increased activities superoxide dismutase, catalase and glutathione but decreases in malondialdehyde levels were recorded at 50 and 100 mg/kg NG compared to control. Findings reveal that NG strengthens the intrinsic antioxidant defence system against streptozotocin-induced diabetic cortical neural damage in lower animals and thus could be exploited as an adjuvant in the management of diabetes and neuropathies especially after validation via clinical trials.
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